Publications

2024

Structural basis of H3K36 trimethylation by SETD2 during chromatin transcription

Jonathan W Markert, Jelly H Soffers, Lucas Farnung

Science, DOI: 10.1126/science.adn6319

STK19 positions TFIIH for cell-free transcription-coupled DNA repair

Tycho ET Mevissen, Maximilian Kümmecke, Ernst W Schmid, Lucas Farnung†, Johannes C Walter†

Cell, DOI: 10.1016/j.cell.2024.10.020

Chromatin Transcription Elongation – A Structural Perspective

Lucas Farnung

Journal of Molecular Biology, DOI: 10.1016/j.jmb.2024.168845

Resolution of transcription-induced hexasome-nucleosome complexes by Chd1 and FACT

Maik Engeholm†, Johann J Roske, Elisa Oberbeckmann, Christian Dienemann, Michael Lidschreiber, Patrick Cramer†, Lucas Farnung†

Molecular Cell, DOI: 10.1016/j.molcel.2024.08.022

In silico screening identifies SHPRH as a novel nucleosome acidic patch interactor

Allison M James*, Ernst W Schmid, Johannes C Walter†, Lucas Farnung†

bioRxiv, DOI: 10.1101/2024.06.26.600687

2023

Structure of the complete S. cerevisiae Rpd3S-nucleosome complex

JW Markert, SM Vos†, L Farnung†

Nature Communications, DOI: 10.1038/s41467-023-43968-8

Nucleosomes unwrapped: Structural perspectives on transcription through chromatin

L Farnung

Current Opinion in Structural Biology, DOI: 10.1021/jacs.2c13512

Structural insights into human co-transcriptional capping

G Garg, C Dienemann, L Farnung, J Schwarz, A Linden, H Urlaub, P Cramer

Molecular Cell, DOI: 10.1016/j.sbi.2023.102690

Structural Basis of Sirtuin 6-Catalyzed Nucleosome Deacetylation

ZA Wang, JW Markert, SD Whedon, MY Abeywardana, K Lee, H Jiang, C Suarez, H Lin, L Farnung†, PA Cole†

Journal Of The American Chemical Society, DOI: 10.1021/jacs.2c13512

2022

Structural basis of nucleosome retention during transcription elongation

M Filipovski, JH Soffers, SM Vos, L Farnung

Science 376 (6599), 1313-1316

Structure of a backtracked hexasomal intermediate of nucleosome transcription

L Farnung*, M Ochmann, G Garg, SM Vos, P Cramer (*Co-corresponding author)

Molecular Cell 82, 3126–3134

Assembly of RNA polymerase II transcription initiation complexes

L Farnung and SM Vos

Current Opinion in Structural Biology 73, 102335

2021

Structural basis of nucleosome transcription mediated by Chd1 and FACT

L Farnung, M Ochmann, M Engeholm, P Cramer

Nature Structural & Molecular Biology 28 (4), 382-387

Mechanism of SARS-CoV-2 polymerase stalling by remdesivir

G Kokic, HS Hillen, D Tegunov, C Dienemann, F Seitz, J Schmitzova, L Farnung, A Siewert, C Höbartner, P Cramer

Nature Communications 12 (1), 1-7

Ruler elements in chromatin remodelers set nucleosome array spacing and phasing

E Oberbeckmann, V Niebauer, S Watanabe, L Farnung, M Moldt, A Schmid, P Cramer, CL Peterson, S Eustermann, K Hopfner, P Korber

Nature Communications 12, 3232

2020

Structure of complete Pol II–DSIF–PAF–SPT6 transcription complex reveals RTF1 allosteric activation

SM Vos, L Farnung, A Linden, H Urlaub, P Cramer

Nature Structural & Molecular Biology 27 (7), 668-677

Nucleosome-CHD4 chromatin remodeller structure maps human disease mutations

L Farnung^, M Ochmann, P Cramer^

eLife 9, e56178: ^Co-corresponding authors

Structure of replicating SARS-CoV-2 polymerase

HS Hillen, G Kokic, L Farnung, C Dienemann, D Tegunov, P Cramer

Nature 584, 154–15

Structure of H3K36-methylated nucleosome–PWWP complex reveals multivalent cross-gyre binding

H Wang, L Farnung, C Dienemann, P Cramer

Nature structural & molecular biology 27 (1), 8-13

2018

Structure of transcribing RNA polymerase II-nucleosome complex

L Farnung, SM Vos, P Cramer

Nature communications 9 (1), 1-6

The interaction landscape between transcription factors and the nucleosome

F Zhu, L Farnung, E Kaasinen, B Sahu, Y Yin, B Wei, SO Dodonova, KR Nitta, E Morgunova, M Taipale, P Cramer, J Taipale

Nature 562 (7725), 76-81

Structure of paused transcription complex Pol II–DSIF–NELF

SM Vos*, L Farnung*, H Urlaub, P Cramer

Nature 560 (7720), 601-606

Structure of activated transcription complex Pol II–DSIF–PAF–SPT6

SM Vos, L Farnung, M Boehning, C Wigge, A Linden, H Urlaub, P Cramer

Nature 560 (7720), 607-612

Cryo-EM structure of a mammalian RNA polymerase II elongation complex inhibited by α-amanitin

X Liu, L Farnung, C Wigge, P Cramer

Journal of Biological Chemistry 293 (19), 7189-7194

2017

Mechanism of RNA polymerase II stalling by DNA alkylation

S Malvezzi*, L Farnung*, CMN Aloisi, T Angelov, P Cramer, SJ Sturla

Proceedings of the National Academy of Sciences 114 (46), 12172-12177

Nucleosome–Chd1 structure and implications for chromatin remodelling

L Farnung, SM Vos, C Wigge, P Cramer

Nature 550 (7677), 539-542

2016

Nucleosomal arrangement affects single-molecule transcription dynamics

V Fitz, J Shin, C Ehrlich, L Farnung, P Cramer, V Zaburdaev, SW Grill

Proceedings of the National Academy of Sciences 113 (45), 12733-12738

2014

The structure and substrate specificity of human Cdk12/Cyclin K

CA Bösken, L Farnung, C Hintermair, MM Schachter, K Vogel-Bachmayr, D Blazek, K Anand, RP Fisher, D Eick, M Geyer

Nature communications 5 (1), 1-14